Background: Pyogenic infections are characterized by severe local inflammation, usually with pus formation which is increasingly being caused by MDR isolates leading to difficulty in treatment and unfavourable outcomes. Objectives: To study bacteriological profile and antimicrobial susceptibility pattern of isolates from pus samples Material and methods: This prospective study was undertaken in the Department of Microbiology, Geetanjali Medical College and Hospital, Udaipur. Processing of samples and identification of isolates were done according to the standard bacteriological protocols. Antimicrobial susceptibility testing, screening and confirmation of phenotypic resistance traits were performed and interpreted based on CLSI guidelines. Results: Total of 143 isolates were obtained from 140 culture positive samples. Male preponderance was seen and most cases were between 21-40 years of age. Maximum resistance in GPC isolates was seen for beta-lactams and beta-lactam combination drugs while in GNB isolates, third and fourth generation cephalosporins were also resistant along with beta lactam drugs. MRSA, ESBL and ICR production were detected in approximately 50-60% isolates. No MBL producing strain was isolated. Conclusions: It is important for microbiology laboratory to record phenotypic traits like MRSA, ICR, ESBL, AmpC and MBL production, as these traits result in multidrug resistant isolates making treatment a challenge. A hospital should take proactive steps in setting up antibiotic policy and AMSP guidelines and constitute a hospital infection committee to monitor the emergence of drug resistance and should implement standard work precautions among health care personnel along with orientation programmes.